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Investigation of Riboflavin binding to DNA using polymerase chain reaction
Author(s) -
Banco Michael,
Benore Marilee Ann
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.975.6
Subject(s) - riboflavin , flavin mononucleotide , cofactor , flavin group , flavin adenine dinucleotide , biochemistry , dna , chemistry , binding site , enzyme , microbiology and biotechnology , biology
Riboflavin is water‐soluble vitamin that is a precursor to the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). It is well documented that Riboflavin, also known as vitamin B 2 , demonstrates affinity for binding to DNA. Evidence also indicates that Riboflavin plays a role in bacterial gene expression, by binding to riboswitch sites. In our lab we are investigating the binding of riboflavin to eukaryotic DNA, in order to evaluate the affinity and specificity of the binding. We are using polymerase chain reaction (PCR) inhibition to characterize the binding of riboflavin to specific sequences in the Riboflavin Binding Protein gene.