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Evaluation of Oxidative DNA Damage, Lipid Peroxidation and Antioxidant Status in Patients Undergoing Surgery with Sevoflurane Anesthesia
Author(s) -
Braz Mariana Gobbo,
Orosz José Eduardo B,
Pierine Damiana T,
Braz Leandro G,
Ferreira Ana Lúcia A,
Salvadori Daisy Maria F,
Yeum KyungJin,
Braz José Reinaldo C
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.974.2
Subject(s) - sevoflurane , lipid peroxidation , malondialdehyde , anesthesia , oxidative stress , antioxidant , genotoxicity , comet assay , medicine , anesthetic , pharmacology , dna damage , chemistry , biochemistry , toxicity , dna
Genotoxicity of volatile anesthetics such as sevoflurane is still controversial. We evaluated the possible effects of sevoflurane on DNA damage, lipid peroxidation and antioxidant status in patients without comorbidities undergoing minimally invasive surgery with general anesthesia. Fifteen patients classified as physical status (ASA) I, aged 18–44 years old, of both genders, and scheduled for septoplasty or tympanoplasty, received anesthesia maintained with 2%–3% of sevoflurane. Blood samples were collected before anesthesia (T0), 15 min after anesthesia induction (T1), 120 min of anesthesia (T2) and on the first postoperative day (T3). DNA damage in lymphocytes was determined by the comet assay using specific enzymes to detect oxidized purines and pyrimidines. Plasma lipid peroxidation (malondialdehyde ‐ MDA) as well as antioxidant capacity were evaluated by a fluorometry. There were no significant differences in DNA oxidized bases and antioxidant capacity (P>;0.05) among the four time points evaluated; however, MDA levels decreased during T2 (P=0.006) in relation to T3. In conclusion, the current study indicates that sevoflurane anesthesia does not induce oxidative stress in patients without comorbidities undergoing minor surgeries, suggesting the safety of this anesthetic regarding genotoxicity. Supported by FAPESP, Brazil #2009/51110–5