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Three‐dimensional reconstructions of human embryos: Morphogenesis and hepatic vascularization.
Author(s) -
LHUAIRE Martin,
SCHARBARG Emeric,
MICARD Émilien,
CENDRE Romain,
DELMAS Vincent,
DUPARC Fabrice,
MERCIER Philippe,
FELBLINGER Jacques,
BRAUN Marc,
AVISSE Claude,
LABROUSSE Marc
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.967.6
Subject(s) - embryo , morphogenesis , trichrome , anatomy , embryology , masson's trichrome stain , immunohistochemistry , portal vein , biology , pathology , medicine , h&e stain , microbiology and biotechnology , radiology , gene , biochemistry
OBJECTIVES The purpose of this study was to describe the morphogenesis of hepatic gland and its vascularization in 4 human embryos using three‐dimensional reconstructions from histological sections, and to compare our results with literature. MATERIALS AND METHODS We investigated four embryos belonging to three prestigious historical French collections of human embryos serially sectioned: 7 mm (Carnegie Stage 14), 15 mm (CS 18), 22.5 mm (CS 21) and 27 mm (CS 23) respectively. Staining included either Masson's trichrome or standard HES. Scanning was performed using an Epson V750 PRO ®, (resolution 3200 dpi). Registration of image stacks and export in DICOM files were performed using original software developed by IADI Laboratory (INSERM U947, NANCY). The images obtained were exploited with ORS ® Visual. RESULTS From the 7mm embryo to 27mm, the hepatic gland was very large and filled almost the entire abdominal cavity. The hepatic vascularization was well visualized and individualized. The portal vein and hepatic veins were distinct from the 15mm embryo to subsequent stages. CONCLUSION This method of three‐dimensional reconstructions of hepatic morphogenesis brings a new perspective to the study of human embryology. Grant Funding Source : AAA