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Empirical development of a histological protocol for whole brain sectioning to characterize neuropathological patterns in human specimens
Author(s) -
Chan Kelly Marissa,
Schmalz Naomi A.,
Choy Kristi,
Nguyen Anthony,
Pham Tracie N.,
Stark Elena,
Dong Hongwei,
Toga Arthur W.,
Vinters Harry V.,
Wisco Jonathan J.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.967.2
Subject(s) - pathology , neuropathology , medicine , protocol (science) , disease , alternative medicine
This project describes the empirical development of a histological protocol for preparing whole brain sections in order to map gross patterns of microinfarctions, deposition of amyloid beta, and neurofibrillary tau tangles. Typical neuropathology biopsy procedures sample areas of the brain restricted in size by the dimensions of small cassettes; thus, histological protocols rarely provide explicit guidelines to address challenges faced when preparing large tissue sections for microscopic observation and image acquisition. Formalin‐fixed, coronal and sagittal human blocks were prepared for paraffin sectioning. These samples exceeded the recommended thickness and area guidelines in existing protocols for typical paraffin‐based procedures, so protocols for processing, embedding, sectioning, and mounting were modified. By deconstructing and defining this process, we ultimately intend to revive this rare practice of whole brain histology and enhance our understanding of the pathogenesis of cerebrovascular and Alzheimer's disease. Grant Funding Source : NIH/NIA, NIH/NCRR, Translational Research Fund (UCLA), McGinty Family Foundation