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Expression and function of tenascin‐C during colorectal enteric nervous system development
Author(s) -
Nagy Nandor,
Akbareian Sophie,
Steiger Casey,
Molnar David,
Barad Csilla,
Goldstein Allan M
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.965.4
Subject(s) - neural crest , enteric nervous system , extracellular matrix , tenascin , microbiology and biotechnology , biology , crest , cell migration , tenascin c , hindgut , anatomy , cell , neuroscience , fibronectin , embryo , biochemistry , physics , botany , quantum mechanics , larva , midgut
The intestinal microenvironment contains several extracellular macromolecules that play important roles during enteric nervous system (ENS) development. Tenascin‐C is an extracellular matrix glycoprotein that regulates cell migration, possibly by modulating the adhesiveness of neural crest cells to their substratum. The role of tenascin‐C in enteric neuronal development is unknown. In this study we show that tenascin‐C is dynamically expressed during ENS development in the chick gut. It is absent from the cecal region prior to the arrival of crest‐derived cells, and then is found to colocalize with the migrating wavefront. Experimentally generated aganglionic hindgut leads to loss of tenascin‐C expression in the submucosal area. Analysis of FACS‐sorted neural crest cells by qRT‐PCR and generation of chick‐rat intestinal chimeras both demonstrate that vagal‐derived enteric neural crest cells produce tenascin‐C, whereas sacral‐derived crest cells do not. Based on vitro migration assays, we find that tenascin‐C promotes the migration of enteric neural crest cells. We conclude that vagal‐derived enteric neural crest cells produce tenascin‐C to promote their migration during ENS development. Grant sponsor: NIH: R01DK080914 (AG); Bolyai Fellowship (NN)