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Coordinated regulation of ZnT1, Zip6 and MT‐1 mRNA in cultured choroid plexus elicited by changes in extracellular zinc and prolactin
Author(s) -
Aquino Mayra,
Young Robin,
Villalobos Alice
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.950.4
Subject(s) - choroid plexus , messenger rna , prolactin , extracellular , metallothionein , prolactin receptor , medicine , zinc , gene expression , endocrinology , receptor , microbiology and biotechnology , chemistry , biology , hormone , biochemistry , gene , organic chemistry , central nervous system
We are investigating regulation of zinc (Zn) exporters (ZnTs), Zn importers (ZIPs) and the Zn‐binding protein metallothionein (MT) in the choroid plexus epithelium as it adapts to changes in Zn availability or select hormones. By real‐time qRT‐PCR, we analyzed ZnT1, Zip6 and MT‐1 gene expression in cultured choroid plexus epithelial cells treated for 48 h with 10 μM DTPA an extracellular chelator or 25 μM ZnCl 2 (n = 3). In Zn‐treated cells, ZnT1 mRNA increased 2‐fold at 3–48 h; MT‐1 mRNA also increased 6‐fold at 3 h, but peaked at 12 h; Zip6 mRNA increased 6‐fold at 12–48 h. Zn chelation reduced ZnT1 mRNA 60% at 3–48 h and reduced MT‐1 mRNA 90% at 6–48 h; however, Zip6 increased 6‐fold at 12–48 h. Prolactin (PRL) increases Zip6 gene expression in mammary epithelia; choroid plexus expresses PRL receptors. Choroid plexus cells were treated for 24 h with 1, 10, 100 and 1000 nM PRL (n = 1). In a concentration‐dependent manner, PRL increased ZnT1 mRNA 30–70%, but increased Zip6 mRNA 3‐ to 6‐fold and increased MT‐1 mRNA 4‐ to 10‐fold. These data indicated coordinated regulation of Zn transporters, e.g. , ZnT1 and Zip6, and MT elicited by changes in Zn availability and in response to prolactin.