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Preventive effect of diurnal endogenous melatonin combined with exercise on the cartilage destruction in collagenase‐induced arthritic rats
Author(s) -
Hong Yunkyung,
Jo Youngjin,
Lee Seunghoon,
Hong Yonggeun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.941.5
Subject(s) - ex vivo , medicine , osteoarthritis , endocrinology , melatonin , circadian rhythm , cartilage , chemistry , anatomy , pathology , biochemistry , alternative medicine , in vitro
Osteoarthritis (OA) is characterized by articular cartilage destruction, subsequent joint space narrowing, and pain. The purpose of the study is to determine effects of endogenous melatonin with exercise on the cartilage break‐down in OA rats. Rats, which were injected with type VII collagenase, were divided into two groups: OA + vehicle (Veh), and OA + exercise (Ex). Treadmill exercise (30min/day, 5 days/week) was initiated at 07:00 (Ex[7AM]), 14:00 (Ex[2PM]), and 22:00 (Ex[10PM]), depending on diurnal melatonin rhythm. Compared with the Veh, reduction was shown in the not joint thickness but width of Ex[2PM] as well as Ex[10PM] rats at 4 weeks (P<0.05). MMP13 mRNA was significantly increased at 1 week (P<0.05). MMP13 mRNA was the highest in the Ex[7AM] at 4 weeks, whereas its expression was the lowest in Ex[10PM] (P<0.05). COL2A1 mRNA was increased in the Ex[10PM], whereas it was decreased in the Ex[7AM] as well as Ex[2PM] rats at 1 week (P<0.05). Compared to unaffected side, the bone volume density of affected joint was increased in the Veh at 4 weeks (P<0.05). Trabecular thickness was increased in the Veh (P<0.05). However, there was no significance for both parameters in the intervention groups. These findings suggest that treadmill exercise has a prophylactic effect on articular cartilage destruction, and it is dependent on diurnal rhythm.