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Muscular Remodeling by Melatonin with and without Therapeutic Exercise in Collagenase‐induced Osteoarthritic Rats
Author(s) -
Kim JooHeon,
Hong Yunkyung,
Lee Seunghoon,
Hong Yonggeun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.939.7
Subject(s) - medicine , ex vivo , osteoarthritis , endocrinology , treadmill , collagenase , gastrocnemius muscle , skeletal muscle , chemistry , pathology , biology , in vivo , biochemistry , alternative medicine , enzyme , microbiology and biotechnology
Osteoarthritis (OA) is characterized by progressive degeneration of arthritic cartilage, subsequent joint narrowing, and abnormal gait. The purpose of this study was to determine the effects of melatonin with and without exercise on muscle remodeling and functional gait in collagenase‐induced OA rats. A melatonin (MT) injection was given subcutaneously twice/day. Treadmill exercise (Ex) was conducted for 1 h/day at a frequency of 5 days/week. Body weight was significantly reduced by MT with and without exercise (P < .05), whereas no significant change was observed in the gastrocnemius or tibialis anterior muscle mass in the OA+MT or OA+MT+Ex groups at day 28. MAFbx, a ubiquitin E3 ligase, was increased in the medial gastrocnemius in OA+MT+Ex rats at day 7 (P < .05). Intervention also caused upregulation of autophagic molecules LC3 and BECN1 (P < .05). At day 28, the relative distribution of MHC1¥â was significantly increased in the gastrocnemius of OA+MT and OA+MT+Ex groups (P < .05). Sarcomeric nebulin was also increased by MT with and without Ex at day 28 (P < .05). Footprint analysis showed that intervention increased the feet contact area, recovering functional gait. The results indicate that muscle remodeling by intervention recovered functional gait in collagenase‐induced OA in rats.