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miRNAs Regulate Skeletal Muscle Development via Down‐regulation of Myosin Heavy Chain
Author(s) -
Chen Xin,
Zhu Minsheng
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.939.12
Subject(s) - myosin , myogenesis , microrna , c2c12 , gene knockdown , myh7 , skeletal muscle , untranslated region , microbiology and biotechnology , biology , myosin light chain kinase , transcription factor , myocyte , chemistry , gene , messenger rna , biochemistry , anatomy
MicroRNAs (miRNAs) are a class of small non‐coding RNAs which suppress gene expression through posttranscriptional binding 3′‐untranslated region (UTR). Some miRNAs have been found mediate muscle differentiation via repressing the transcription factor or signal molecule in myogenesis. However, the functional role and molecular mechanism by which miRNAs regulate muscle differentiation via contractile protein are not understood. Myosin II is a family of actin‐binding proteins and it¡s the important structure protein for muscle contraction. Using luciferase reporter assay and western blot, we revealed miR‐23a directly inhibited muscle myosin heavy chain (MyHC) expression, while miR‐199a‐5p depressed level of non‐muscle myosin heavy chain IIA (NMHC IIA) protein. Augmentation or reduction of the two miRNAs either inhibited or promoted myogenic differentiation in C2C12 cell line, respectively. Furthermore, knockdown of muscle MyHC or NMHC IIA with siRNA also impaired muscle differentiation. These data suggest a novel microRNA‐myosin mechanism during muscle differentiation.