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Myocardial AT1 gene expression is increased in a model of left ventricular hypertrophy induced by high salt intake.
Author(s) -
Pereira Rafael Canavel,
Katayama Isis Akemi,
Heimann Joel Claudio
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.936.3
Subject(s) - medicine , endocrinology , glyceraldehyde 3 phosphate dehydrogenase , losartan , ventricle , renin–angiotensin system , gene expression , angiotensin ii receptor type 1 , muscle hypertrophy , angiotensin ii , left ventricular hypertrophy , chemistry , gene , blood pressure , biochemistry
Objective To evaluate gene expression of the renin‐angiotensin system components in a model of left ventricular hypertrophy induced by high salt intake demonstrated in a previous study. Methods Wistar rats were fed normal (NS1.3% NaCl) or high (HS 8%) salt diet since weaning. From the 7th week of age, two HS subgroups received hydralazine or losartan (HZ 15 and LOS 20mg/kg/day). Angiotensinogen, renin, ACE, ACE2, AT1 and AT2 gene expression in left ventricle were measured. Results (n=8/group)NS HS HS+HZ HS+LOSAngiotensinogen/GAPDH (IDV) 0.21±0.017 a 0.19±0.017 a 0.21±0.013 a 0.19±0.024 aRenin/GAPDH (IDV) 0.71±0.02 a 0.68±0.02 a 0.67±0.02 a 0.67±0.02 aACE/GAPDH (IDV) 0.87±0.03 a 0.83±0.05 a 0.97±0.06 a 097±0.05 aACE2/GAPDH (IDV) 0.41±0.02 a 0.43±0.02 a 0.37±0.03 a 0.34±0.02 aAT1/GAPDH (IDV) 0.23±0.00 a 0.46±0.06 b 0.45±0.06 b 0.22±0.00 aAT2/GAPDH (IDV) 0.52±0.04 a 0.51±0.03 a 0.45±0.03 a 0.44±0.02 aData are reported as mean±SEM. Means in row with superscripts without a common letter differ, P<0.05Conclusion High salt intake increases AT1 gene expression, however it is prevented by losartan treatment. Supported by FAPESP and CNPq.