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Central Sympathoinhibition Ameliorates the Reduction of Splenic Regulatory T Cells with Decreased IL‐17 Production in Hypertensive Rats
Author(s) -
Katsuki Masato,
Hirooka Yoshitaka,
Kishi Takuya,
Sunagawa Kenji
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.927.2
Subject(s) - spleen , foxp3 , medicine , endocrinology , blockade , losartan , downregulation and upregulation , blood pressure , receptor , immunology , angiotensin ii , chemistry , immune system , biochemistry , gene
Background Regulatory T cells (Tregs) have been reported to prevent organ damages in hypertension. This effect might be caused by anti‐inflammatory process. We examined Treg expression and number focusing on spleen where T cells are abundant. The aim of the present study was to determine whether Tregs is altered in the spleen, and blockade of brain Ang II type 1 receptors (AT 1 R) for central sympathoinhibition affects splenic Tregs in hypertensive rats. Methods and Results Stroke‐prone spontaneously hypertensive rats (SHRSP) with sympathetic hyperactivity were treated with intracerebroventricular infusion of an AT 1 R blocker (losartan, 1mg/kg/day for 14 days, ARB) or vehicle (VEH). Wister Kyoto rats (WKY) served as control. Splenic Foxp3 (an essential transcription factor of Tregs) mRNA and CD4 + CD25 + Foxp3 + cells were lower in SHRSP‐VHE than in WKY (Foxp3 mRNA: 0.47±0.03 vs. 1.02±0.03, P<0.05). Splenic Foxp3 mRNA expression levels were upregulated in ARB‐SHRSP (ARB: 0.44±0.04 vs. VEH: 0.31±0.02, P<0.05), while systolic blood pressure and 24‐hour urinary norepinephrine excretion were decreased. IL‐17 mRNA in the spleen was significantly lower in SHRSP‐ARB than in SHRSP‐VEH (ARB: 0.27±0.04 vs. VEH: 0.47±0.03, P<0.01). Conclusion Central sympathoinhibition induced by blockade of brain AT 1 R ameliorates the reduction of splenic Tregs possibly via decreased IL‐17 production in SHRSP.