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Phenylephrine‐induced calcium oscillations in human mesenteric arterial smooth muscle
Author(s) -
Tejerina Teresa,
NavarroDorado Jorge,
Fameli Nicola,
GarciaAlonso Mauricio,
Ramajo Marta,
Urraca Concha,
Redondo Santiago,
Medina Ursula,
Breemen Casey
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.923.1
Subject(s) - myograph , phenylephrine , cyclopiazonic acid , vasoconstriction , mesenteric arteries , vascular smooth muscle , chemistry , nifedipine , medicine , contraction (grammar) , calcium , blood vessel , endocrinology , artery , biophysics , smooth muscle , biology , blood pressure
Phenylephrine (PE)‐induced oscillatory fluctuations in intracellular Ca 2+ concentration ([Ca 2+ ] i ) of vascular smooth muscle (SM) have been observed in every blood vessel isolated from a wide variety of mammals. Paradoxically, until recently similar observations in humans have proven elusive. In this study, we report for the first time observations of adrenergically‐stimulated [Ca 2+ ] i oscillations in human mesenteric artery SM. We partially characterized these oscillations by using the inhibitory drugs nifedipine, 2‐aminoethoxydiphenyl borate (2‐APB), cyclopiazonic acid (CPA), and HA‐1077. PE and one of these drugs were added to different arterial segments, which had previously been loaded with Fluo‐4 AM in PSS and mounted on a myograph on the stage of a confocal microscope. We observed PE‐induced oscillations in [Ca 2+ ] i , which initiated and maintained vasoconstriction. After PE contraction, all drugs provoked relaxation of the vessel segments, sometimes only partial, and reduced or inhibited oscillations, except CPA, which rarely caused relaxation. These preliminary results point to a potential involvement of the sarcoplasmic reticulum Ca 2+ and IP3R in the maintenance of the observed oscillations. FIS PI080920 , RECAVA RD06/0014/1007