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Effects of NO/cGMP inhibitors in a rat model of anaphylactoid shock
Author(s) -
Fagundes Agnes Afrodite Sumarelli Albuquerque,
Carvalho Marco Túlio Menezes,
Ferreira Luciana Garros,
Silveira Ana Paula Cassiano,
Nadai Tales Rubens,
Capellini Verena Kise,
Evora Paulo Roberto Barbosa,
Celotto Andrea Carla
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.920.7
Subject(s) - agonist , medicine , shock (circulatory) , blockade , compound 48/80 , chemistry , pharmacology , anesthesia , receptor , degranulation
Anaphylaxis is a severe clinical manifestation of allergic diseases. The aim was investigate the effect of NO/cGMP pathway inhibition with L‐NAME and methylene blue (MB) and indigo carmine (IC, α‐adrenergic receptor agonist) in C48/80‐induced shock in rats. Animals were allocated in groups: Control, C48/80, MB, MB+C48/80, C48/80+MB, L‐NAME, L‐NAME+C48/80, C48/80+L‐NAME, IC, IC+C48/80 and C48/80+IC. The drugs were administered 5 min. before or after the C48/80. The systolic blood pressure (SBP) and the survival were analyzed every 10 min. over a period of 60 min. Administration of C48/80 decreased the SBP (89±5 to 27±7 mmHg), led to cyanosis and killed 60% of the animals within 60 min. Plasma NOx levels were increased in C48/80 group compared to Control. The use of MB and L‐NAME prior to shock induction does not prevented the SBP reduction (37±7 and 43±8 mmHg respectively), but increased the survival in 50%. When MB and L‐NAME were administered after C48/80, no improvements in SBP were observed and survival was reduced. The IC before or after C48/80 not inhibited the decrease in SBP and in both groups the survival was lower than C48/80 group. In this rat model of anaphylactoid shock there is an increase of NO production, but the blockade of NO/cGMP pathway did not restores the SBP. However, these blockers increased animal survival. IC not inhibited the decrease in SBP and not prevented animals death by shock. Supported by FAEPA.