Premium
Interactions between L‐arginine supplementation and physical training in the mitochondrial biomarkers and nitric oxide bioavailability in rat skeletal muscle
Author(s) -
Zanesco Angelina,
Silva Carmem V,
Delbin Maria A,
La Guardia Paolo G,
Davel Ana P,
Priviero Fernanda B
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.920.6
Subject(s) - nitric oxide synthase , endocrinology , medicine , gastrocnemius muscle , nitric oxide , arginine , superoxide dismutase , skeletal muscle , chemistry , tfam , aerobic exercise , citrate synthase , nitrite , biochemistry , mitochondrion , biology , oxidative stress , enzyme , mitochondrial biogenesis , nitrate , amino acid , organic chemistry
Nitric oxide (NO) and physical exercise regulate mitochondrial function with improvement of resistance to fatigue and muscular wasting. We studied the relationship between physical performance and the protein expressions of mitochondrial function markers (mtTFA and ATP synthase subunit c) in gastrocnemius muscle from exercised rats supplemented with L‐arginine during 8 weeks. We also analyzed the nNOS/NO pathway by measuring the content of tissue nitrite/nitrate as well as proteins expression of Cu/Zn superoxide dismutase (Cu/Zn‐SOD), neuronal nitric oxide synthase (nNOS) and mitochondrial manganese superoxide dismutase (Mn‐SOD) in the cytoplasm and mitochondrial fractions from skeletal muscle. Wistar rats were divided into: control sedentary (SD), sedentary supplemented with L‐arginine (SDLA), trained (TR) and trained supplemented with L‐arginine (TRLA). L‐arginine supplementation‐plus physical training promotes an up‐regulation of the protein expressions of ATP synthase subunit c, nNOS, Cu/Zn‐SOD, Mn‐SOD and mtTFA in gastrocnemius muscle that was associated with improvement in fatigue resistance and reduction in body weight. Our data shows that run physical training in combination with L‐arginine supplementation initiate a sequence of biochemical events in isolated gastrocnemius muscle that culminate in improvement of fatigue resistance as well as in lowering body weight gain.