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Evaluating the role of interstitial ang‐II and ATP in insulin induced hypoglycemia by chronic renal microdialysis
Author(s) -
Prathipati Priyanka,
Quadri Syed,
Jackson Debra W,
Jackson Keith E
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.909.16
Subject(s) - microdialysis , interstitial fluid , tubuloglomerular feedback , medicine , endocrinology , insulin , blood pressure , chemistry , renal function , angiotensin ii , hypoglycemia , dopamine
Interstitial metabolic changes in organs can be monitored in real time by microdialysis, a minimally invasive method. Various endogenous and exogenous analytes such as ATP and angiotensin II (Ang‐II) can be continuously collected from multiple sites of the same animal without any fluid loss. Previous studies demonstrated the importance of ATP in the tubuloglomerular feedback (TGF) mechanism and few would argue the importance of AngII in the regulation of blood pressure. Emerging evidence has shown the relation between Ang‐II, heme oxygenase‐1(HO‐1) and blood pressure. Since ATP plays a paracrine role in regulating hemodynamic function, our current study was performed to evaluate the hypothesis that renal derived ATP plays a significant role in maintaining elevated AngII levels during hypertension. Microdialysis probes were inserted into the renal cortex of anesthetized male Sprague Dawley rats (200–225g). Post surgery rats were treated with insulin (7U/kg body weight) or vehicle for 2 weeks. Food and water intake were monitored daily and dialysate samples were collected after insulin dosing and analyzed for ATP and Ang‐II by luciferin‐luciferase assay and ELISA respectively. The present study is the first of its kind to adapt microdialysis for chronic collection of renal interstitial samples and to demonstrate the ability of hypoglycemia to promote the development of hypertension.(Support: BOR Comp 2010).