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SHB deficient mice display an increased GFR and augmented renal arteriolar contractions to both Adenosine and Ang II
Author(s) -
Gao xiang,
Sällström Johan,
Ma Zufu,
Welsh Michael,
Persson Erik
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.909.14
Subject(s) - tubuloglomerular feedback , chemistry , adenosine , angiotensin ii , medicine , endocrinology , renal function , afferent arterioles , kidney , receptor
BACKGROUND Src homology 2 domain‐containing protein B ( Shb ) is an adapter protein which regulates several signal transduction cascades and endothelial cell functions. Adenosine‐angiotensin II (Ado‐Ang II) interaction plays important roles in regulation of glomerular filtration rate (GFR), vascular resistance and tubuloglomerular feedback. We used Shb ‐knockout ( Shb −/− ) and wild‐type ( Shb +/+ ) mice to investigate GFR and effectiveness of Ado and Ang II to renal resistance vessels. METHODS GFR was measured in conscious Shb −/− and Shb +/+ mice using FITC‐inulin. Isolated and perfused renal afferent arteriolar responses to Ang II doses (10 −12 to 10 −6 M) or low‐dose Ado (10 −8 mol/l) alone, as well as Ado (10 −8 mol/l) in combination with Ang II doses were studied from both genotypes. RESULTS GFR was increased significantly in Shb −/− (371 ± 12 μL/min) comparing to Shb +/+ (321 ± 11 μL/min). The maximal arteriolar contraction to Ang II was significantly larger in Shb −/− (87 %) than in Shb +/+ (54 %). Low‐dose Ado alone contracted afferent arterioles (6% in Shb −/− ; 7% in Shb +/+ ) and significantly enhanced Ang II arteriolar constrictions (6% in Shb −/− ; 18 % in Shb +/+ ). CONCLUSION Low‐dose adenosine augments Ang II arteriolar constriction effectiveness, which indicates Ado‐Ang II interaction in both Shb −/− and Shb +/+ mice. The absence of Shb increases GFR and enhances renal arteriolar contractions to both Ado and Ang II.