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Intrinsic balance of small artery active and passive diameter‐tension relations
Author(s) -
Tuna Bilge Guvenc,
Bakker Erik NTP,
VanBavel Ed
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.902.6
Subject(s) - distension , tension (geology) , anatomy , isometric exercise , chemistry , biomedical engineering , in vivo , materials science , medicine , biology , composite material , ultimate tensile strength , microbiology and biotechnology
Arterial remodeling is important in the pathogenesis of vascular diseases and target organ damage. In this study we induced matrix remodeling to smaller diameters and studied active diameter‐tension relations to understand the link between active and passive biomechanics. Diameter‐tension relations were obtained in the passive state and upon full activation (125 mM K + + 10 −6 M NE) by wire myography before and after matrix cross linking by incubation with transglutaminase 2 (1, 5 hours TG2, 50μg/ml, n=6) or after low flow induced remodeling by ligation of rat mesenteric small arteries (1 day LF1, 3days LF3, n=8). The diameter for peak force capacity (Dopt, control 289±6, TG2 258±9 * , LF1 258±12 * , LF3 247±5 * ) shifted in the same direction as the passive diameter at 100 mmHg equivalent pressure (D100, control 353±9, TG2 317±17 # , LF1 302±13 # , LF3 301±7 # ), normalizing Dopt/D100 balance in vitro and in vivo. Active force at large distention fell simultaneously with increased passive force in all groups. Only LF3 vessels had increased contractile capacity at small diameters. In conclusion, active tension capacity at high distension is masked by passive tension, challenging the concept that active and passive elements are arranged simply parallel, and suggests that there is an intrinsic mechanism linking biomechanical properties of active and passive components.