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Drag reducing polymers diminishing the near vessel wall cell poor region
Author(s) -
Brands Judith,
Kameneva Marina,
Pacella John
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.902.12
Subject(s) - perfusion , saline , cremaster muscle , microcirculation , in vivo , medicine , chemistry , blood flow , red blood cell , biology , microbiology and biotechnology
Drag reducing polymers (DRP) are blood soluble long‐chain macromolecules that have been shown to enhance microvascular perfusion during severe coronary stenosis. The mechanism for the enhanced perfusion is not clear. Recently, it was discovered that nanomolar concentrations of DRP in red blood cell (RBC) suspensions flowing in a straight microchannel significantly reduce the near wall cell poor region (CPR) thickness. Therefore, we hypothesized that DRP redistribute RBCs within the vasculature reducing the CPR, which may in turn enhances perfusion. In rat cremaster tissue, CPR was measured in arterioles (range 25–44 μm) at baseline and 5, 15, 25 and 35 minutes after administration of DRP (blood concentration of 1ppm, N=5) or saline (control, N=6). In the control group, there was a 20% increase in CPR compared to baseline 15–35 minutes after infusion of saline (P<0.05). After DRP, CPR was significantly reduced compared to the CPR measured in the control group (72% of control measurement) and reduced to 87% compared to corresponding baseline at all time points (P<0.05). These data demonstrated that in vitro observations showing a decrease in CPR do occur in vivo , and that redistribution of RBC within the microvascular network may contribute to DRP perfusion enhancing effects via an increase in wall shear stress and a reduction in plasma skimming. Supported by AHA 12POST9400001 and NIH K08 1 K08 HL086730–01