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Stability of shunt flow pathways following thermal injury confirmed by multi‐wavelength laser speckle contrast imaging of oxy‐hemoglobin.
Author(s) -
Frame Mary Molly,
Dewar Anthony M,
Hartig Renee,
Islam Farha,
Zhou NaiYun,
Du Congwu
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.898.8
Subject(s) - shunt (medical) , microcirculation , blood flow , medicine , chemistry , anesthesia
Our goal was to determine the stability of flow shunt pathways causing increased heterogeneity of flow with inflammation. Thermal injury (50C thermocouple controlled 300um spot burn) was used as a non‐pharmacological means to create a zone of inflammation with control regions in the same tissue. Intravital microscopy of the cheek pouch in male hamsters (N=10, isoflurane) was viewed. Rhodamine labeled RBC confirmed shunt pathways through all terminal arteriolar networks (NETS) within 500um of a spot burn (burn zone, paired locations before v 15m‐2hr after). Flow was redirected along arcades away from the burn zone. Oxy‐and deoxy‐hemoglobin (HbO 630nm v HbR 570nm) was measured (multi‐wavelength laser speckle contrast imaging) across a 2×2cm area containing the burn. Total Hb was consistent with tube hematocrit of 5–40%. Locations within 500um of the burn rim (near) v further away (far) show HbO was 30% less in perfused large arterioles (>;50um) near v far from the burn, and 24% less in perfused large venules near vs far. Within NETS far from the burn, HbO was 7% higher along the shunt vs the nonshunt pathway. Near the burn, HbO is 71% greater along the shunt vs the non‐shunt pathway. Comparing HbO along shunt pathways, HbO was decreased 52% near vs far from the burn rim. Data with HbO or RBC flux agreed. Thus, a diminished oxygen shunt pathway is initiated by thermal injury and maintained for several hours. AHA 0655908T