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The effects of brief ischemia and reperfusion on interstitial PO 2 in the rat spinotrapezius muscle
Author(s) -
Dodhy Sami C.,
Golub Aleksander S.,
Pittman Roland N.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.898.18
Subject(s) - ischemia , oxygen , anesthesia , chemistry , reperfusion injury , oxygenation , blood flow , medicine , cardiology , organic chemistry
The involvement of oxygen in reactive hyperemia following brief periods of local flow arrest was investigated by measuring PO 2 under conditions of different ischemic durations of 5, 15, 30, 60, and 300 sec. Spinotrapezius muscles of anesthetized male Sprague‐Dawley rats were used; the muscle was covered with a gas barrier film to isolate it from atmospheric oxygen. Well perfused sites were chosen with one or more arterioles and a capillary network. Phosphorescence quenching microscopy was used to measure interstitial PO 2 with a topically applied phosphorescent probe. Localized ischemia was produced by rapidly inflating an airbag over the muscle; reperfusion was initiated by deflating the bag. Baseline PO 2 varied between 52 and 60 mmHg. During ischemia, PO 2 dropped to 45 mmHg for 5 s ischemia down to 3 mmHg for >;30 s ischemia. Maximum PO 2 during the reperfusion phase ranged from 52 to 71 mmHg. The time required for PO 2 recovery to baseline ranged from 9 (5 s ischemia) to 122 sec (300 s ischemia). These data exhibited a positive correlation between ischemic duration and the time for PO 2 recovery, as well as to the maximum PO 2 reached during reperfusion. The data will be used to establish a relationship between ischemic duration and tissue oxygen consumption (VO 2 ) during reperfusion. Support: NHLBI grant HL18292