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Effect of microsphere encapsulation on peptide clearance from the respiratory tract
Author(s) -
McKenicole Renae
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.893.2
Subject(s) - microsphere , plga , encapsulation (networking) , clearance , chemistry , peptide , respiratory tract , drug delivery , pharmacology , nanotechnology , nanoparticle , respiratory system , medicine , biochemistry , materials science , chemical engineering , urology , computer science , computer network , engineering
Microspheres harbor great potential as an effective means of targeted delivery to specific tissues for use in cancer treatment and vaccinations. Both encapsulation volume and size were examined in this research. Microspheres were created by the encapsulation of EP67‐FITC in Poly Lactic‐co‐Glycolic Acid (PLGA). Different sizes (360–370 nm and 8.8 ìm) and volumes (15 ìl and 50 ìl of the same 50ìg dose) of microspheres were investigated to determine their effect on localization and clearance in the respiratory tract of female BALB‐c mice. Results indicate that the PLGA encapsulated EP67‐FITC was more rapidly cleared than unencapsulated EP67‐FITC. Also, the 50 ìl volume of unencapsulated EP67‐FITC localized to the stomach of the mice while encapsulated EP67‐FITC did not. This research shows a clear advantage to PLGA encapsulation and additionally to smaller particles (360–370 nm). This publication was made possible by grants from the National Center for Research Resources (5P20RR016469) and the National Institute for General Medical Science (NIGMS) (8P20GM103427), a component of the National Institutes of Health (NIH) and its contents are the sole responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH.