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Cyclodextrin‐complexed Lippia grata essential oil reduce c‐fos expression in the periaqueductal gray area following the antinociceptive effect in mice
Author(s) -
Lima Pollyana Siqueira,
Lucchese Angélica,
Quintans Jullyana Siqueira,
Souto Natália Barreto Rocha,
Cardoso Roberta Santana Deodório,
Alexandria Santos Darlisson,
Alves Péricles Barreto,
Lucca Waldecy,
Menezes Paula dos Passos,
Souza Araújo Adriano Antunes,
QuintansJúnior Lucindo José
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.893.1
Subject(s) - periaqueductal gray , nociception , chemistry , pharmacology , lippia , capsaicin , olfactory bulb , hyperalgesia , anesthesia , essential oil , medicine , central nervous system , biochemistry , receptor , midbrain , chromatography
Lippia grata Schauer (Verbenaceae) leaves essential oil/β‐cyclodextrin (EO/β‐CD) complex was used to evaluated if the complex formulation is able in reduces orofacial behavior in mice. Male Swiss mice were pretreated with EO/β‐CD (50, 100 and 200 mg/kg, p.o., per os , orally), morphine (5 mg/kg, i.p.) or vehicle (distilled water). Ninety minutes after the treatment, we injected formalin (20 μL, 2%), capsaicin (20 μL, 2.5 μg) or glutamate (40μL, 25 μM) into the right upper lip (perinasal area). For the action in the CNS, 90 minutes after the treatment, the animals were perfused, the brains collected, and submitted in an immunofluorescence protocol for Fos protein. Experimental protocols were approved by the Animal Care and Use Committee at the UFS (# 57/11). EO/β‐CD produced significant (p< 0,05; p<0.01) antinociceptive effect, in the formalin‐, capsaicin‐and glutamate‐induced orofacial nociception. The immunofluorescence showed that the EO/β‐CD activated significantly (p<0.05) the olfactory bulb, the piriform cortex, and the periaqueductal grey of the CNS. Together, our results provide first‐time evidence that EO/β‐CD attenuates orofacial nociception at least, in part, through an activation of CNS areas, such as periaqueductal grey.