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Ethanol increases expression of macrophage metalloelastase (MMP‐12) via NADPH oxidase‐dependent ROS production in RAW264.7 macrophages
Author(s) -
Kim Mi Jin,
Nepal Saroj,
Park PilHoon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.890.22
Subject(s) - nadph oxidase , chemistry , gene knockdown , ethanol , reactive oxygen species , microbiology and biotechnology , oxidase test , biochemistry , enzyme , biology , apoptosis
The present study was undertaken to investigate the role of NADPH oxidase in ethanol‐induced MMP‐12 expression in RAW 264.7 macrophages. Here we found that ethanol induces significant increase in expression of NADPH oxidase subunits via p38 MAPK/NF‐κB pathway. Furthermore, knockdown of NOX2 (gp91 phox ), a membrane subunit of NADPH oxidase, by siRNA significantly inhibited ethanol‐induced ROS production, suggesting a critical role of NADPH oxidase in ROS production by ethanol. Ethanol treatment also causes significant increase in MMP‐12 expression at both mRNA and protein level. Inhibition of NOX2 by siRNA or by a selective pharmacological inhibitor prevented ethanol‐induced MMP‐12 expression. Taken together, these results demonstrated that NADPH oxidase‐derived ROS production plays a pivotal role in MMP‐12 expression in RAW 264.7 macrophages.