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Antioxidant effect of phycobiliproteins on proteins and DNA exposed to a reactive oxygen species generating system.
Author(s) -
OlveraRamirez Roxana,
Estrada_Perez Alan,
Alcalde_Vazquez Raul,
Muñoz Jose Luis
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.890.15
Subject(s) - phycobiliprotein , reactive oxygen species , antioxidant , oxidative stress , biochemistry , ascorbic acid , chemistry , dna , cyanobacteria , food science , biology , bacteria , genetics
It is well known that cyanobacteria has been used as antioxidants in some toxicological models, as well as fluorescent markers and as natural pigments in food industry, but little is known about its role in oxidative stress protection. The aim of this study is to assess the possible protective effect of phycobiliproteins (PBP's) on proteins (BSA) and DNA exposed to a reactive oxygen species (ROS) generating system. A raw extract of PBP's was obtained from the cyanobacteria Arthrospira maxima and was used like antioxidant. A series of tubes with Bovine seroalbumine (BSA) were exposed to 10 μl of Cu (II) and 100 μL of ascorbic acid like a ROS generating system, also plasmidic DNA PGLO was exposed. Using spectrophotometry was demonstrated that BSA and DNA were affected. Another series of tubes were incubated under the same conditions but were added with different concentrations of the PBP's raw extract. The results showed that PBP's exhibit some protective effect against oxidative damage using 30 μL from FBP's extract in BSA and 100 μL in plasmidic DNA. Also was demonstrated that DNA plasmidic electrophoretic pattern was conserved when the PBP's extract was added.