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Interdependent expression of P2X receptors in the mouse kidney: P2X4‐P2X7 receptor “cross‐talk”
Author(s) -
Birch Rebecca,
ScottWard Toby,
Tam Frederick W K,
PeppiattWildman Claire M,
Unwin Robert J,
Wildman Scott S P
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.884.3
Subject(s) - kidney , receptor , messenger rna , purinergic receptor , reabsorption , hek 293 cells , medicine , microbiology and biotechnology , chemistry , biology , andrology , endocrinology , gene , biochemistry
In the renal collecting duct (CD), an ionotropic P2X4‐like receptor may act as a luminal Na sensor, regulating ENaC‐mediated Na reabsorption [1]. Several P2X receptor subunits are expressed in the CD and evidence is emerging that P2X4 and P2X7 subunits might interact [2]. P2X7 is also linked to many renal pathologies. Here we determine whether P2X4 and P2X7 interact at the expression level in the CD. Using target‐specific primers, we quantified P2X4, P2X7 (and β‐actin) mRNA levels in mouse kidney and microdissected CD of wild‐type (WT), P2X4−/− and P2X7−/− mice by real‐time RT‐PCR. Expression of P2X4 and, to a lesser extent P2X7 mRNA was readily detected in WT kidney (n=3), whereas P2X4 and P2X7 mRNA levels were <100‐fold lower in P2X4−/− and P2X7−/− mice respectively (n=3). Expression of P2X4 mRNA was reduced in P2X7−/− kidney (P=0.03; n=3), and a substantial decrease in P2X7 mRNA was observed in the CD of P2X4−/− mice (P<0.05; n=3). Our studies indicate that expression levels of P2X4 and P2X7 in the collecting duct are interdependent and suggest a P2X4/7 heteromeric assembly as the luminal Na sensor. Research supported by the Wellcome Trust