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Immunohistochemical localization of arginase‐1 expression in B6 and BALB/c mice
Author(s) -
Meyerholz David K
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.874.6
Subject(s) - arginase , pathology , immunostaining , immunohistochemistry , biology , spleen , lymphatic system , medicine , immunology , arginine , biochemistry , amino acid
Arginase is the final enzyme of the urea cycle hydrolyzing L‐arginine to ornithine and urea. The isoform arginase‐1 has been suggested to play a role in cardiovascular disease, cystic fibrosis, asthma and cancer. The goal of this study was to develop and validate immunohistochemical detection of arginase‐1 in formalin‐fixed paraffin embedded mouse tissues from B6 and BALB/c strains. Immunohistochemistry was performed using a primary Ab (polyclonal rabbit anti‐arginase‐1) with optimization/validation using the liver, where zone 1–2 hepatocytes express arginase‐1, but zone 3 lacks cellular expression. Tissues with prominent arginase‐1 cellular expression included liver, endocrine pancreas, lymphoid tissues, esophagus, skin, lung, thyroid, parathyroid, bone marrow, intestine, spleen, salivary glands and clitoral glands. Various disease states were also examined. Normal lymphoid cells in spleen had moderate diffuse arginase‐1 expression, but neoplastic lymphocytes lacked arginase‐1 expression. Hepatocytes, in a liver infiltrated by lymphoma, had enhanced arginase‐1 expression. Lungs with eosinophilic crystalline pneumonia commonly had alveolar macrophages with strong immunostaining. Endothelial cells of vessels had common immunostaining, while erythrocytes and platelets were negative. Smooth muscle of various tissues had multifocal uncommon immunostaining. There was no evidence of strain‐dependent differences in arginase‐1 expression. The diversity of tissues and cells that expressed arginase‐1 emphasizes the potential importance of arginase‐1 in disease models.