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The Engraftment Problem: Identifying Proteins that Mediate Stem Cell Adhesion
Author(s) -
Prisco Anthony,
Hoffmann Brian R,
Kaczorowski Catherine C,
Greene Andrew S
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.874.2
Subject(s) - progenitor cell , microbiology and biotechnology , cell adhesion , adhesion , cytokine , stem cell , bone marrow , endothelial progenitor cell , immunology , chemistry , biology , organic chemistry
Many methods exist to treat heart failure; however no current treatment induces new vessel or cardiomyocyte formation. Recently, researchers have used bone marrow derived endothelial progenitor cells (EPCs) to reverse heart failure in rodents. Unfortunately, results from human studies using similar approaches have been inconsistent. One reason is that only a small percentage of injected cells ultimately engraft into damaged tissue. The first step in engraftment following an IV injection of EPCs is binding of the EPC to the vascular endothelium (VE) within the damaged tissue. Data from our lab has demonstrated that low doses of TNFα (a pro‐inflammatory cytokine) increases the adhesion of EPCs to VE cells in vitro . We hypothesize the protein pairs responsible for mediating the EPC/VE interaction are differentially regulated by TNFα and are located on the surface of the cell. The goal of this study was to identify proteins responsible for mediating EPC/VE adhesion. To identify protein pairs we conducted a novel membrane targeted proteomic based analysis on both EPCs and VE cells treated with TNFα. Candidates were identified based upon stringent, predetermined criteria. Our analysis revealed a high‐confidence list of candidate proteins that are likely responsible for mediating EPC/VE adhesion.