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Ω‐3 Fatty Acid Effects on Obese Leptin Knock‐Out Mice
Author(s) -
Ali Arfaa,
Nachnani Jagdish,
Bulchandani Deepti,
Alba Laura,
Mansour Ahmad,
Hamdan Hana,
Quinn Tim,
Molteni Agostino,
Herndon Betty
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.872.6
Subject(s) - steatosis , medicine , endocrinology , leptin , fatty liver , corn oil , leptin receptor , adiponectin , fatty acid , histopathology , diabetes mellitus , biology , chemistry , obesity , insulin resistance , pathology , biochemistry , disease
Liver steatosis in wild‐type rats on methionine‐choline deficient diets is decreased with fish oil ( Nachnani, Diabetologia 53:153,2010 ). Effects of Ω‐3 fatty acid were studied here in OB/OB (leptin k/o) mice with natural obesity and steatosis. Ten mice received purified Ω‐3 (20% EPA, 50% DHA) in food pellets, equivalent (mg/kg) to a human dose of ~4 grams/day. Ten more OB/OB mice received corn oil in their food. At 60 day necropsy, livers were harvested for study. Five Ω‐3 treated mice survived and unexpectedly blood glucose of >;600 mg/dL was found in 3 of them. Since leptin receptor k/o mice (DB/DB) are diabetes models, liver homogenate and sera of Ω‐3 and controls were analyzed for murine leptin receptor (ELISA), but no difference was found between groups. Ω‐3 treated mice showed reduced fatty liver infiltration, 27.6% Ω‐3 and 38.5% untreated, with less periportal and portal triad inflammation in Ω‐3 mice. Heart histopathology showed irregular, eosinophilic myocardial fibers with pyknotic nuclei, suggesting necrosis. Such changes in heart cell morphology is reported in human diabetes. Sera showed significant lowering of insulin, IL‐6, adiponectin, and TNFα in the Ω‐3 group compared to untreated. Ω‐3 fatty acid treatment may reduce liver steatosis in OB/OB mice but other physiological changes with diabetes induction may limit its use as a model. Funded: St. Luke's Foundation, Kansas City