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Sex‐Dependent Depot Differences in MMPs and Inflammation of Adipose Tissue Remodeling in Mice
Author(s) -
Wu Yuanyuan,
Lee MiJeong,
Fried Susan K.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.865.12
Subject(s) - adipose tissue , mmp3 , endocrinology , medicine , matrix metalloproteinase , population , biology , stromal cell , adipocyte , white adipose tissue , depot , gene expression , gene , biochemistry , environmental health , history , archaeology
We reported (Grove et al, IJO, 2010) gonadal (GON) and inguinal (ING) sc adipose tissues of high fat (HF) fed obese female compared to male mice (C57/BL6) are less inflamed. Microarray and RT‐qPCR studies revealed significant sex and depot differences in the expression of metalloproteinases (MMPs), enzymes that remodel adipose tissue in obesity. We followed up by comparing the mRNA expression of MMPs, 3 and 12, and their inhibitors (TIMPs) in age‐matched mice fed low compared high fat (45% calories) diets for 14 weeks, and extended studies to the mesenteric, a visceral depot and dorsal Sc (DSC), which in contrast to the ING, does not include mammary gland. As expected, HF diets induce a robust inflammatory response that includes increases in MMP12 in males, but not females and the response was greater in the visceral than sc depots. In females, in contrast, MMP3 mRNA was higher in sc than visceral (both ING and DSC) with low fat (LF) feeding and was not affected by the HF diet. However, HF diet increased MMP3 in all depots in males. MMP3 and MMP12 were mainly expressed in the stromal cell fraction. Flow cytometry and qRT‐PCR analysis suggested MMP3 transcripts were made from Lin − CD29 + Sca1 + CD34 +/− population enriched for preadipocyte markers, while MMP12 was expressed in macrophages. We conclude that there are major sex dependent depot differences in gene expression of MMPs and TIMPs and those differences may contribute to sex‐ and depot‐differences in adipocyte size and adipose tissue expansion. Funding sources: Evans Center for Interdisciplinary Biomedical Research and NIH(R24DK087669).