Premium
Maternal Dietary Canola Oil Suppresses Mammary Carcinogenesis in Rat Offspring
Author(s) -
Park Chung Sun,
Mabasa Lawrence,
Cho Kyongshin,
Walters Mark William
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.863.9
Subject(s) - canola , offspring , carcinogenesis , biology , mammary tumor , endocrinology , mammary gland , medicine , breast cancer , cancer , pregnancy , genetics , botany
The objective was to investigate the effect of maternal dietary canola oil on the susceptibility of female offspring to mammary carcinogenesis. Pregnant Sprague‐Dawley rats were fed either a control diet or diet containing 10% canola oil. At weaning, mammary tissues were collected for analyses of mammary development genes. Dams were then replaced by their offspring for the mammary tumorigenesis experiments. At 50 days of age, mammary carcinogenesis was induced in the offspring by an intraperitoneal injection of N‐nitroso‐N‐methylurea. Tumor tissues were collected for analysis of genes by quantitative real‐time PCR and protein expression by Western blot. The offspring of canola oil‐fed dams showed a 15% reduction in tumor incidence. These canola diet‐offspring also exhibited significantly decreased tumor multiplicity (47%) and tumor volume (80%). While canola oil diet did not change the mRNA expression of beta casein, acetylcoenzyme A carboxylase alpha, and tumor suppressor 53, transcription of gamma‐glutamyltransferase 1 was significantly higher in the canola dams and offspring. Results suggest a potential protective effect of maternal dietary canola oil on mammary cancer risk of the offspring. These findings may be important in devising maternal nutritional strategies to reduce long term breast cancer risk in humans. Supported by the USDA‐NIFA and the Northern Canola Growers Association.