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Green tea prevents aberrant crypt foci (ACF) in mice fed the total Western diet (TWD) but not the AIN93G diet
Author(s) -
Hintze Korry Joseph,
Ward Robert,
Benninghoff Abby,
Lefevre Michael
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.863.6
Subject(s) - biology , food science , nutrient , zoology , ecology
The new TWD emulates average American intakes (at the NHANES 50th percentile) for micro and macronutrients based on nutrient density. Mice were fed either TWD or AIN93G basal diets supplemented with or without 0.2% green tea extract in the drinking water. Green tea extract reduced bodyfat percentage in both the TWD and AIN93G groups (P < 0.05) and decreased fasting glucose levels in mice fed TWD (P = 0.02) but not AIN93G. Mice fed TWD without green tea had more ACF and a higher total crypt cell count compared to cohorts fed the AIN93G diet (P < 0.001). Interestingly, green tea extract decreased ACF and total crypt cells in the TWD group (P < 0.001) but not in mice fed the AIN93G basal diet. Cecal bacterial DNA from each treatment was pyrosequenced and a metagenomic analysis of the microbiome will be presented. These results suggest that the Western dietary pattern promotes carcinogenesis and that supplementation with chemopreventive bioactives, such as green tea, may be beneficial to populations consuming a poor diet. Funded by the Utah Ag Experiment Station.