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Lycopene inhibits the proliferation and progression of human colon cancer cells in both in vitro and in vivo models
Author(s) -
Tang FengYao,
Pai ManHui,
Chiang EnPei Isabel,
Wang XiangDong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.863.12
Subject(s) - lycopene , proliferating cell nuclear antigen , colorectal cancer , cancer research , in vivo , immunohistochemistry , chemistry , in vitro , cell growth , cancer , pathology , medicine , biology , biochemistry , carotenoid , microbiology and biotechnology
The current study demonstrated that lycopene significantly inhibited the proliferation of human colon cancer cells in vitro. Moreover, consumption of lycopene prevented the growth and progression of colorectal tumor in a mouse xenograft model. Bioluminescence imaging, histopathological, immunofluorescence (IFC), and immunohistochemical (IHC) staining results indicated that lycopene effectively suppressed the growth and progression of colon cancer. The results demonstrated that lycopene significantly suppressed the nuclear expression of PCNA and beta‐catenin proteins. Consumption of lycopene augmented the E‐cadherin adherent molecule and nuclear levels of cell cycle inhibitor p21CIP1/WAF1 protein. The chemopreventive effects of lycopene were associated with suppression of COX‐2, PGE2, and phosphorylated ERK1/2 proteins. Furthermore, the inhibitory effects of lycopene were inversely correlated with the plasma levels of matrix metalloproteinase 9 (MMP‐9) in tumor‐bearing mice. These results suggested that lycopene could act as a chemopreventive agent against the growth and progression of colorectal cancer in a mouse xenograft model.