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Yerba‐Mate ( Ilex paraguariensis ) extract prevents the liver against ethanol‐induced liver injury in rats.
Author(s) -
KojimaYuasa Akiko,
Tamura Akiko,
Sasaki Mio,
Yamashita Haruka,
Saku Taro,
Hikima Tadamasa,
Tabuchi Masaki,
Munakata Hiroshi,
MatsuiYuasa Isao
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.862.6
Subject(s) - cyp2e1 , ethanol , liver injury , chemistry , oxidative stress , pharmacology , reactive oxygen species , alcoholic liver disease , biochemistry , medicine , cytochrome p450 , metabolism , cirrhosis
Yerba–Mate is popular tea beverage produced and consumed in South America. Ethanol metabolism‐associated oxidative stress contributes to the pathogenesis of alcoholic liver disease. The objective of this study was to reveal the protective effect of a Yerba–Mate tea extract on ethanol‐induced liver injury. Methods Isolated hepatocytes were cultured with or without 100 mM ethanol. An extract of Yerba ‐ Mate tea (EMT) was added to the culture medium with ethanol simultaneously. Male Wistar rats were fed a diet with or without 0.005% or 0.02% EMT. Animals were given drinking water containing ethanol 5% (v/v) together with two doses of CCl 4 (0.1 ml/kg BW, i.p.) weekly for 3 weeks. Results In the cultured hepatocytes, EMT treatment suppressed the ethanol‐induced increase in cell death by inhibiting cytochrome p450 2E1 (CYP2E1) activity, which is related to the production of reactive oxygen species. We examined the effects of EMT on serum ALT activity and the progression of liver fibrosis in rats treated with ethanol and CCl 4 . EMT treatment suppressed plasma ALT activity in the ethanol‐ and CCl 4 ‐treated rats. Furthermore, EMT treatment decreased CYP2E1 expression and increased ADH expression in the ethanol‐ and CCl 4 ‐treated rats. EMT treatment protected the rats against ethanol‐ and CCl 4 ‐induced liver injury. Conclusion EMT may be a candidate for preventing ethanol‐induced liver injury.