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Anti‐inflammatory activity and metabolism of α‐mangostin by cells of human origin
Author(s) -
Gutierrezorozco Fabiola,
Chitchumroonchokchai Chureeporn,
Lesinski Gregory B,
Failla Mark L
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.862.4
Subject(s) - garcinia mangostana , xanthone , sulfation , glucuronidation , chemistry , metabolism , cell culture , bioavailability , biochemistry , monocyte , pharmacology , biology , traditional medicine , in vitro , immunology , medicine , microsome , stereochemistry , genetics
Xanthones in mangosteen ( Garcinia mangostana ), a fruit native to Southeast Asia, have been reported to exhibit anti‐proliferative and pro‐apoptotic activities. Recent data from our laboratory suggest ingested xanthones and their metabolites are absorbed and delivered to tissues where they may be accumulated and further metabolized. Neither the anti‐inflammatory activity of xanthones for cells of human origin nor the metabolism of these compounds by animal or human cells have not been examined with the exception of our previous studies with Caco‐2 human intestinal cells. We have found that ≤15 μM α‐mangostin (α‐MG), the most abundant xanthone in mangosteen, suppresses secretion of a) IL‐8 by activated human Caco‐2 intestinal, HT‐29 colonic, and THP‐1 macrophage‐like cell lines, and b) TNFα by activated HepG2 and human blood monocyte‐derived macrophages. α ‐ MG was taken up and metabolized by the various cell types and results with Caco‐2 suggest bioavailability may be enhanced during inflammatory conditions. Furthermore, unconjugated xanthones were predominant in HepG2, THP‐1 and HT‐29 cultures in the pro‐inflammatory environment. In addition to glucuronidation/sulfation of α ‐ MG, conversion to 9‐hydroxycalabaxanthone, garcinones C and D, and other unknown xanthone compounds was observed. Ongoing studies are examining anti‐inflammatory activity of α ‐ MG using a preclinical model. Grant Funding Source : OSU FIC and Conacyt Fellowship (Mexico)