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Quercetin decreases tumorigenesis in a mouse model of breast cancer
Author(s) -
Steiner Jennifer,
Davis J Mark,
McClellan Jamie,
Murphy Angela
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.862.30
Subject(s) - quercetin , carcinogenesis , inflammation , cancer , breast cancer , mammary gland , medicine , tumor necrosis factor alpha , endocrinology , macrophage , cancer research , biology , in vitro , biochemistry , antioxidant
Quercetin is a flavonol with anti‐inflammatory actions in macrophages; however no studies have investigated the benefits of quercetin (Quer) on tumorigenesis and inflammation in a mouse model of breast cancer (BrCa). Female C3(1)/SV40Tag mice were assigned to a Quer (0.2%) or control (AIN‐76A) diet (n=14–15) at 4wks of age and tumor number and volume were recorded bi‐weekly. At 20wks mice were sacrificed; tumors and mammary glands were analyzed for mRNA and protein expression. Quer significantly decreased tumor number (20wks) and volume (18.5–20wks); at sacrifice (20wks) average tumor volume was 2061.8 ± 977.0 mm3 in control mice and only 462.9 ± 75.9 mm3 with quercetin treatment. This was consistent with a reduction in invasiveness and numbers of ductal lesions in the mammary gland. Plasma MCP‐1 and tumor MCP‐1 protein was decreased with quercetin (P<0.05). Similarly, markers of pro‐tumor macrophages (CD206 and IL‐10) were reduced in the mammary gland of quercetin treated mice (P<0.05). However, tumor mRNA expression of inflammatory cytokines, MCP‐1, IL‐6, and TNF‐alpha, and the macrophage marker, F4/80, did not differ between treatments. These data provide strong support for a beneficial effect of quercetin on inflammatory processes however, it is likely that inflammation‐independent pathways are also involved. Grant Funding Source : COBRE grant from NIH/NIGMS (1P20GM103641–01)