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Effects of supplementation of a cranberry extract enriched in A‐type proanthocyanidins on intestinal bacterial composition of pigs fed obesogenic diets
Author(s) -
Monagas Maria,
Molokin Aleksey,
Lakshman Sukla,
Bruna Gonzalo,
Urban Joseph,
SolanoAguilar Gloria
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.862.28
Subject(s) - proanthocyanidin , bifidobacterium , feces , food science , composition (language) , lactobacillus , biology , weight gain , gut flora , polyphenol , zoology , body weight , microbiology and biotechnology , endocrinology , biochemistry , fermentation , antioxidant , linguistics , philosophy
Proanthocyanidins, which are amongst the most bioactive polyphenols in our diet, are catabolized by intestinal microbiota. The effect of cranberry extract containing ~7% proanthocyanidins (CEPA), on weight gain and composition of intestinal microbiota was evaluated. Young pigs fed 14 mg of CEPA/kg /day added to a basal (10% kcal from fat) or high fat (HF) (35% kcal from fat) diet for 12 weeks were compared to pigs fed similar diets without CEPA. DNA from fecal samples, collected at day 0 and weeks 2, 4, 6, 10, and contents from proximal and distal colon collected at necropsy were processed. Feeding CEPA decreased body weight gain only in pigs fed a basal diet. Total bacteria measured by RT‐PCR, was not affected by the diet or CEPA. Certain Lactobacillus species (spp) were significantly increased in feces from pigs fed the HF diet (3.2–20 fold) at weeks 4 and 6, and in proximal and distal contents. CEPA supplementation significantly increased (2 fold) the abundance of certain Lactobacillus spp at week 4 in both basal and HF diets. The abundance of E. coli spp increased (3–20 fold) in pigs fed the HF diet starting at week 2, and in the proximal and distal contents. In contrast, Bifidobacterium spp were decreased (42 fold) in distal contents of pigs fed the HF diet. In summary, these results showed that CEPA consumption did not induced major changes in certain bacterial species that may explain reduction in weight gain.