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Impact of broccoli on non‐alcoholic liver disease and cancer in mice fed a Western diet
Author(s) -
Chen YungJu,
Myracle Angela D.,
Wallig Matthew A.,
Jeffery Elizabeth H.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.861.18
Subject(s) - liver cancer , hepatocellular carcinoma , medicine , cancer , fatty liver , inflammation , endocrinology , gastroenterology , colorectal cancer , obesity , disease
Liver cancer is a lethal cancer with a 5‐year survival around 14%. Obesity causes chronic inflammation, associated with an increased risk for liver cancer in men. Our previous study showed that broccoli can decrease both IL‐6 expression and cancer incidence in a model of inflammation‐enhanced colon cancer. Here we used a diethylnitrosamine (DEN)‐induced hepatocellular carcinoma (HCC) mouse model, plus a Western diet high in saturated fat and sucrose as a model for obesity‐enhanced liver development, to evaluate the impact of broccoli on inflammation and subsequent liver cancer. Male C57BL/6J mice (15 days old) were given 25 mg/kg DEN i.p. and fed ad libitum a control (C), Western (W) or Western+10% broccoli (W+B) diet. Mice (n=6) were killed every month. Non‐alcoholic fatty liver disease (NAFLD), altered cellular foci, adenoma and HCC were evaluated histologically; liver triglycerides (TG) and serum alanine aminotransferase (ALT) levels were also evaluated. Serum ALT, increasing over time, was significantly lower in mice receiving the W+B diet compared to those receiving the W diet at month 5. Dietary broccoli decreased NAFLD, but had no overt protective effect on tumor incidence or growth. The project is funded by NCI 5RO3CA162539–02 .