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Astragalin inhibits allergic asthma through modulating oxidative stress‐responsive MAPK signaling
Author(s) -
Cho InHee,
Kang YoungHee
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.861.13
Subject(s) - astragalin , oxidative stress , mapk/erk pathway , reactive oxygen species , pharmacology , hacat , chemistry , p38 mitogen activated protein kinases , immunology , medicine , signal transduction , antioxidant , biochemistry , flavonoid , kaempferol , in vitro
Allergic asthma is airway obstruction and chronic inflammation and is triggered by inhaled allergens such as dust mite allergen and pollen. Since inflammation is often associated with an increased generation of reactive oxygen species (ROS), and the biochemical environment in the asthmatic airways is favorable for ROS‐mediated reactions, it can be assumed that oxidative stress is a mechanistically‐imperative factor in asthma. Astragalin (kampferol‐3‐O‐glucopyranoside), a flavonoid with anti‐cancer, antimicrobial properties, has been isolated from tea seeds. This study elucidated whether oxidative stress activated allergic asthmatic responses in airway epithelial BEAS‐2B cells, which was suppressed by treating 1–20 μM astragalin. When airway epithelial cells were exposed to 2 μg/ml LPS, nontoxic astragalin at 1–20 μM suppressed LPS‐induced ROS production, evidenced by fluorescent DCFH‐DA staining. Astragalin attenuated LPS‐enhanced expression of PKCβ2 and p22phox and H 2 O 2 ‐upregulated phosphorylation of JNK and p38 rapidly within 30 min. In contrast, the phosphorylation of Akt and ERK increased due to astragalin treatment. Additionally, astragalin attenuated LPS‐ or H 2 O 2 ‐induced eotaxin‐1 protein expression of epithelial cells. Therefore, dietary astragalin can be effective in ameliorating allergic airway diseases through modulating oxidative stress‐responsive MAPK signaling pathway.