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Genetic variation within the selenoproteome influences anxiety‐related behaviors in zebrafish ( Danio rerio )
Author(s) -
Benner Maia J,
Hardy Ronald W,
Settles Matt L,
Robison Barrie D
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.860.9
Subject(s) - zebrafish , danio , biology , gene , microarray , anxiety , genetics , population , selenium , gene expression , psychology , medicine , chemistry , psychiatry , environmental health , organic chemistry
Selenium (Se) is an essential trace element for brain function, and Se deficiency is associated with neurodegenerative diseases. Polymorphisms in the selenoproteome (Se‐dependent proteins that mediate the biological effects of Se) influence individual requirements and responses to Se. We tested the hypothesis that variation within the selenoproteome influences the behavioral response to Se. We fed four zebrafish strains diets varying in the level of Se (control, Se deficient, and Se supplemented). We measured anxiety behaviors, whole body Se concentration, and selenoproteome gene expression in the diencephalon using both microarrays and Real Time PCR. Se concentration increased linearly with increasing dietary Se for all strains. We observed sex and population dependent responses to Se in anxiety behaviors and transcription of selenoproteome genes. The transcriptional results were often characterized by hormesis, in which high and low Se levels produced similar deviations from control levels. We are now using RNASeq to identify genetic polymorphisms in the zebrafish selenoproteome that may mediate response to Se. Research support included NIH COBRE, NCGR INBRE, NSF IOS, and the University of Idaho Student Grant Program. Grant Funding Source : NIH COBRE, NCGR INBRE, NSF IOS, and the University of Idaho Student Grant Program