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Intestinal resolution: helminth parasite clearance through selenoproteins
Author(s) -
Nelson Shakira M,
Urban Joseph,
Prabhu K Sandeep
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.860.10
Subject(s) - nippostrongylus brasiliensis , parasite hosting , biology , macrophage , inflammation , helminths , immunology , microbiology and biotechnology , in vitro , biochemistry , world wide web , computer science
Selenium (Se) in the form of selenoproteins, imparts many beneficial health and anti‐inflammatory properties. Using the tRNA [Ser]Sec (Trsp) KO mice macrophages that lack expression of selenoproteins, we previously demonstrated a complete abrogation of alternatively (M2) activated macrophage activity under high Se (250 nM) conditions, emphasizing the role of selenoproteins in resolution. Current in vivo studies, using the helminthic parasite Nippostrongylus brasiliensis , showed Se supplementation significantly increased intestinal M2 marker expression, while decreasing intestinal worms and fecal eggs. To implicate macrophage‐specific selenoproteins in resolution, Se supplemented WT fl/fl mice, compared to Trsp fl/fl Cre LysM mice, showed a complete abrogation in M2 marker expression with a significant increase in intestinal worms and fecal eggs. Moreover, inhibition of the COX pathway using indomethacin displayed stunted expression of M2 markers despite high Se levels. These data suggest that optimal Se status in the form of selenoproteins, and selenium‐produced anti‐inflammatory prostaglandins are critical to skew macrophage activation to promote resolution of (parasite) infection. NIH grant DK077152 Grant Funding Source : NIH