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Analysis of antioxidant activity of silicon in vitro and murine macrophages
Author(s) -
Choi MiKyeong,
Kim EunJin,
Bu SoYoung,
Sung MiKyung,
Jo ChaeWon,
Kang Myunghwa
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.859.1
Subject(s) - antioxidant , dpph , scavenging , chemistry , hydroxyl radical , nitric oxide , nuclear chemistry , in vitro , reagent , radical , biochemistry , food science , organic chemistry
The purpose of this study was to investigate the antioxidant activity of silicon (Si) in vitro. The antioxidant activity of Si was investigated by using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical‐scavenging, hydroxyl radical scavenging, hydrogen radical scavenging and SOD‐liked activity. Also murine macrophage cell line (RAW 264.7) was utilized to determine the effect of Si on the production of nitric oxide. DPPH radical scavenging activity was 16.7% in 10 uM, 18.0% in 50 uM and 20.0% 100 uM of Si. SOD‐liked activity was 20.5% in 50 uM, 28.0% in 100 uM and 40.9% in 10 mM of Si. Hydroxyl radical scavenging activity of Si was not found at a low concentration but the effect was found at a concentration of 1 M of Si. Hydrogen radical scavenging activity was 24.2% in 25 uM, 26.1% in 50 uM, 29.9% in 100 uM and 29.3% in 10 mM of Si. Macrophages were plated for 6 hours, stimulated with either LPS (10ng/ml) as an inflammatory reagent or LPS with Si (1, 5, 10, 25, 50 uM), doses of which does not affect cell viability. By 24 and 48hrs treatment, Si dose‐dependently decreased the LPS‐induced nitric oxide production. As results, we conclude that Si has an antioxidant property in test tube and biological condition. “This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011–0010880)”