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Treadmill exercise training modulates PCSK9 metabolism in high fat fed mice
Author(s) -
Wen Shin,
Jadhav Kavita S,
Williamson David L,
Rideout Todd C
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.857.6
Subject(s) - pcsk9 , medicine , endocrinology , aerobic exercise , kexin , lipid metabolism , chemistry , treadmill , ldl receptor , metabolism , lipoprotein , cholesterol
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a novel biomarker of LDL clearance and is a potential therapeutic target of cardiovascular disease risk. Our objective was to examine the effects of aerobic exercise training in modulating PCSK9 metabolism and hepatic sterol regulation in a high fat fed C57Bl/6 mouse. Mice (n=8) were randomly assigned to a high fat diet (HF) or a high fat diet with exercise (HFE) group for 8 weeks. The HFE group was progressively trained 5 days/week on a motorized treadmill (up to 5% grade, 45 min/day, 26 m/min). Endpoint bodyweight was significantly reduced (p<0.05) in the HFE group compared with the HF animals (48.36±1.60 vs 41.13±1.04 g). Aerobic training reduced (p<0.05) plasma LDL/VLDL and PCSK9 concentrations by 41 and 31%, respectively, compared with the HF group. Compared with the HF group, hepatic PCSK9 mRNA was increased (~2 fold, p<0.05) in the HFE group without a corresponding change (p>;0.05) in protein abundance. Aerobic exercise was further associated with an increase in hepatic LDLr mRNA (~1.4 fold, p<0.05) but no change (p>;0.05) in total tissue LDLr protein abundance was observed compared with the HF group. These results suggest that treadmill exercise modulates hepatic and blood PCSK9 metabolism and may underlie exercise‐induced lipid responses in high fat fed C57Bl/6 mice. Grant Funding Source : UB start up funds