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Differential regulation of gene expression by α‐Tocopherol and α‐Tocopheryl‐phosphate in pre‐adipocytes and adipocytes
Author(s) -
Lirangi Melania,
Meydani Mohsen,
Zingg JeanMarc,
Azzi Angelo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.857.3
Subject(s) - lipotoxicity , adipocyte , adipogenesis , endocrinology , medicine , lipid droplet , chemistry , inflammation , tocopherol , lipid metabolism , microbiology and biotechnology , biology , insulin resistance , adipose tissue , vitamin e , diabetes mellitus , biochemistry , antioxidant
Adipocytes play an important role in lipid and energy homeostasis and dysregulation of these processes contributes to obesity and type 2 diabetes mellitus. In addition, adipocytes influence inflammation and play a major role in preventing lipotoxicity by removing excess fatty acids from the circulation and storing them as triglycerides and thus reducing exposure of other cells to their potentially harmful effects. Although the many aspects of metabolic disorders associated with lipotoxicity and obesity cannot be fully clarified with cultured cells, the NIH3T3‐L1 preadipocytes/adipocytes differentiation model has been useful to study the molecular processes involved. In this model, several vitamins and phytochemicals have been shown to influence proliferation and differentiation. Here, we report by using quantitative RT‐PCR that in NIH3T3‐L1 pre‐adipocytes, α‐tocopheryl phosphate (αTP) (but not α‐tocopherol (αT)) activates a set of genes [TRB3 (Tribbles Homolog 3), Sestrin‐2, and Insulin Induced Gene 1 (INSIG)] potentially preventing lipids accumulation. In contrast, in differentiated adipocytes, αTP inhibits transcriptional expression of the same genes, potentially facilitating lipid uptake and storage. Thus, in proliferating pre‐adipocytes αTP foils lipid accumulation, whereas in adipocytes it enhances lipid accumulation. Supported by USDA Contract #58–1950‐0–014.