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Effects of fasting on adipose stress and inflammation
Author(s) -
Torchon Tania,
Wong Kristen,
Ji Bo,
Das Suchita,
Spence Jason,
Voy Brynn
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.856.3
Subject(s) - endocrinology , medicine , adipose tissue , white adipose tissue , resistin , lipolysis , pdk4 , adipose tissue macrophages , adipocyte , chemerin , chemistry , inflammation , downregulation and upregulation , adipokine , biology , insulin resistance , insulin , biochemistry , gene
Fasting activates adipose tissue lipolysis and increases local levels of nonesterified fatty acids, which can alter adipocyte metabolism and inflammation by activating cell surface (TLR4) and nuclear (LXR and PPAR) receptors. We recently reported that an acute fast (5 hr) upregulated inflammatory signaling and fatty acid oxidation in white adipose tissue, using the domestic chicken as a model. Our objective was to determine if similar adipose response to fasting is observed in mice. The effect of an overnight (16 hr) fast on lipolysis, inflammation and fatty acid oxidation in perigonadal and inguinal adipose tissue was assessed in 8–10 week‐old male C57BL/6J mice. Multiplex adipokine assays (Milliplex®, Millipore.com ) revealed that fasting significantly increased levels of plasminogen activator inhibitor‐1 (PAI‐1) in serum and in abdominal and inguinal adipose tissue (p<0.05). Leptin concentration and resistin levels in plasma but not adipose tissue were significantly decreased by fasting (p<0.05). Fasting significantly increased expression of pyruvate dehydrogenase kinase 4 ( Pdk4 ), which enhances fatty acid oxidation by inactivating pyruvate dehydrogenase. Western blot analyses indicate that fasting upregulated p38MAPK stress kinase signaling. Collectively, these data indicate that fasting activates select inflammatory pathways that may mediate metabolic adaptations to energy deprivation. Grant Funding Source : UT AgInnovation