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Regulation and specificity of the Chd1 chromatin remodeler
Author(s) -
Bowman Gregory,
Patel Ashok,
Chakravarthy Srinivas,
Nodelman Ilana
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.84.3
Subject(s) - chromodomain , nucleosome , chromatin , atpase , microbiology and biotechnology , dna , chromatin remodeling , chemistry , biology , biophysics , enzyme , biochemistry , gene , helicase , rna
Chromatin remodelers are ATP‐dependent enzymes required for the active reorganization of nucleosomes. Accompanying the conserved ATPase is a broad diversity of auxiliary domains that define different remodeler subfamilies, and presumably help guide the outcome of remodeling reactions. We discovered that the double chromodomain unit of the Chd1 remodeler regulates activation of the ATPase motor by blocking access to DNA. Disruption of the chromo‐ATPase interface results in a loss of specificity for nucleosomes over naked DNA, allowing for robust DNA‐dependent ATPase stimulation. Interestingly, this regulatory interface also appears to dampen the nucleosome sliding activity of the remodeler. We are investigating the stages at which chromodomain regulation impacts nucleosome sliding, and the potential impact of this regulation on substrate selection.