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Extracts of sweet potato leaves protect β cells from oxidative stress‐mediated injury
Author(s) -
Lin ChiaYu,
Chiang TzuYuan,
Huang PeiJane,
Li PoJun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.834.2
Subject(s) - oxidative stress , chemistry , antioxidant , catalase , superoxide dismutase , reactive oxygen species , hydrogen peroxide , glutathione peroxidase , biochemistry , glutathione , dpph , intracellular , enzyme
Pancreatic β‐cells are very sensitive to oxidative stress due to relatively low in the expression of antioxidant enzymes, which might play an important role in β‐cell death in diabetes. In the present study, we investigated whether the extracts of sweet potato leaves (SPL) have protective effects against hydrogen peroxide‐induced damage in INS‐1 pancreatic β‐cells. Total phenolic compounds, scavenging activity against DPPH radicals, reducing power method, and ferric chelating ability were evaluated for the antioxidant ability of SPL samples. INS‐1 cells were treated with SPL and/or H 2 O 2 for various concentrations from 2mg/ml to 0.5mg/ml for 24 hours. The reduced cell proliferation after H 2 O 2 toxicity is significantly recovered in the presence of SPL as evaluated by MTT assay (p<0.05). The H 2 O 2 ‐induced overproduction of intracellular reactive oxygen species is greatly inhibited by SPL extracts (p<0.05), suggesting the free radical scavenging activity of SPL. In parallel, extracts of SPL increase activities of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase in H 2 O 2 treated INS‐1 cells (p<0.05). In summary, these results suggest that SPL might be used as potential nutraceutical agent which will protect the pancreatic β‐cells from oxidative stress associated with diabetes.