Premium
ARHGEF3.2 modulates Wnt‐PCP signaling in dorsal marginal zone of Xenopus embryos during early development
Author(s) -
Yoon Jaeho,
Heo JeeIn,
Kim Sung Chan,
Park JaeBong,
Lee JaeYong,
Kim Jaebong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.831.9
Subject(s) - convergent extension , gastrulation , wnt signaling pathway , rhoa , xenopus , microbiology and biotechnology , mesoderm , biology , dishevelled , cdc42 , epiboly , small gtpase , rac gtp binding proteins , signal transduction , embryo , frizzled , embryogenesis , rac1 , embryonic stem cell , genetics , gene
Rho guanine nucleotide exchange factor (RhoGEF) regulates various cellular processes including cell polarity, cytokinesis, and cell movements via activating small GTPase such as RhoA, Rac1 and CDC42. During gastrulation of Xenopus embryos, noncanonical Wnt signaling is the major signal pathway to regulate convergent and extension cell movements in DMZ region. In previous publications, activin induces dorsal mesoderm and noncanonical Wnt signaling is highly activated in dorsal mesoderm to promote cell movements. Although about 70 GEF distinct GEF homologues have been studied in humans, few GEFs of Xenopus are only identified and explored for its function during early embryogenesis. In this study, we showed that EST gene (assesion No. XL.3374), which has been named xARHGEF3.2 was induced by activin treatment in ectodermal explants and expressed at dorsal marginal region in gastrula embryo. It was constructed to explore the role of xARHGEF3.2 during gastrulation. Over‐expression / knockdown of ARHGEF3.2 caused gastrulation defects. Furthermore, Biochemical analysis showed that xARHGEF3.2 activated RhoA through interaction with Dishevelled and Daam‐1. Taken together, our results suggest that xARHGEF3.2 is induced by activin signaling and modulates convergent and extension cell movement via Wnt‐PCP signaling during the early Xenopus embryos development