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The differential effects of carnosol in regulation of HaCaT cell apoptosis upon high or low dose of UVB irradiation
Author(s) -
Tong Lingying,
Wu Shiyong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.831.19
Subject(s) - hacat , apoptosis , chemistry , phosphorylation , cancer research , pharmacology , biochemistry , biology , in vitro
Carnosol, a dietary diterpene extracted mainly from rosemary and sage, was previously reported to have an anti‐cancer effect on breast and prostate cancer by interacting with estrogen and androgen receptors. However, little is known of its effect on carcinogenesis and signaling pathways upon ultraviolet (UV) irradiation. In this report, we showed that the effect of carnosol on apoptosis of HaCaT cells was dependent on the dose of UVB irradiation. Upon a higher dose of UVB irradiation (50 mJ/cm2), carnosol protected the cells from apoptosis. Moreover, we showed that carnosol inhibited the UVB (50 mJ/cm2)‐induced elevation of nitric oxide (NO) and peroxynitrite (ONOO‐). The reduction of NO and ONOO‐was correlated with a partial inhibition of UVB‐induced reduction of IκB, activation and phosphorylation of NF‐κB as well as phosphorylation of JNK. However, upon lower dose of UVB irradiation (6 mJ/cm2), carnosol promoted apoptosis and inhibited recovery of the irradiated cells. Meanwhile, there was no detectable effect on phosphorylation of NF‐κB or JNK upon the lower dose of UVB irradiation and carnosol. Our results suggest that carnosol may be involved in different pathways in regulation of cell death induced by different doses of UVB irradiation. This work is funded by NIH RO1 CA086928 (to S.W.)