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Tropical Cellulolytic Bacteria Isolated From Hindgut Of A. Evuncifer Potential Candidates for Bioconversion of Lignocellulosic Waste Biomass
Author(s) -
IGWOEZIKPE MIRIAM NWANNA,
NwahiriOgbu Chika,
Oyebamiji Olayemi,
Ndukwe Nelly,
Ilori Matthew O.,
Ogbunugafor Henrietta A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.829.4
Subject(s) - bioconversion , lignocellulosic biomass , food science , chryseobacterium , enterobacter cloacae , bacteria , biomass (ecology) , energy source , biology , microorganism , microbiology and biotechnology , chemistry , botany , fermentation , agronomy , biochemistry , ecology , genetics , 16s ribosomal rna , escherichia coli , renewable energy , klebsiella pneumoniae , gene
Studies into the use of lignocellulosic biomass as alternative low cost energy source are on the increase, this has thus necessitated the search for cellulolytic microorganisms to be employed in bioconversion of lignocellulosic materials. This study investigated the degradation of lignocellulosic materials and glucose production potential of bacteria isolated from the hindgut of wood‐eating worker termites Amitermes evuncifer collected from two locations in Lagos, Nigeria. The hindguts of A. evuncifer were inoculated into sterilized cellulolytic media containing wood pulp as sole carbon source and incubated at 37 0C for 14 days. Bacteria were isolated and identified as Bacillus subtilis , Bacillus cereus , Enterobacter cloacae , Enterobacter aerogene , Acinetobacter sp . and Chryseobacterium sp . There was variability in the presence and occurrence of the isolates between the localities. However, the isolates showed zones of clearance on wood pulp agar plates which suggested their utilization of cellulose as the sole carbon and energy source. In addition, growth of B. subtilis , B. cereus , E. cloacae , E. aerogene , Acinetobacter sp . and Chryseobacterium sp . on wood pulp yielded 77.89 ± 1.12, 54.83 ± 1.28, 29.40 ± 2.98, 38.94 ± 2.12, 22.25 ± 1.42 and 61.99 ± 2.52 (mgL‐1) glucose respectively. This study demonstrated the use of tropical cellulolytic bacteria isolates from hindgut of A. evuncifer as potential candidates for bioconversion of lignocellulosic waste biomass.