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Stromal galektin‐1 and myofibroblasts are reponsible for invasive behavior of the head and neck squamous cell carcinoma
Author(s) -
Fik Zdenek,
Valach Jarolsav,
Smetana Karel,
Strnad Hynek,
Chovanec Martin,
Gabius Hans Joachim
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.829.3
Subject(s) - stromal cell , cancer associated fibroblasts , head and neck squamous cell carcinoma , immunohistochemistry , pathology , tissue microarray , myofibroblast , cancer research , head and neck cancer , cancer , tumor microenvironment , biology , tumor progression , stroma , medicine , tumor cells , fibrosis
Cancer‐associated fibroblasts (CAFs) play a significant role in the cross‐talk between tumor storma and the tumor cells. CAFs frequently express smooth muscle actin and are capable to support the developement of invasive cancer cells, which are responsible for establishing of metastases. We set the immunohistochemical presence of smooth muscle actin‐positive (SMA) CAFs in relation to galectin‐1. To pinpoint further correlations on the molecular level, microarray analyses was applied to the transcription profiles of the corresponding tumors, focusing on genes, representing poor prognosis for patient. In the squamous cell carcinomas of head and neck, upregulation of galectin‐1 was significantly correlated to presence of SMA‐positive CAFs in the tumor. Significant correlations of several transcripts were detected in the tumor epithelium with the protein level of galectin‐1 in the CAFs (MAP3K2, SPIN1, etc.). These results provide new insights into the significance of presence of myofibroblasts in squamous cell carcinoma and shows relationship between stromal reactivity and gene expression, associated with cancer progression directly in the samples of head and neck squamous carcinomas. This work was supported by the project PRVOUK No. 27–1 and GAUK No. 291811 of the Charles university.